Mittlere Strasse 69, 4056 Basel, Switzerland

Tel-FAX: +41 61 381 5022

Mobile phone: +41 78 813 5183


PolyLC Switzerland, Europe, Germany, Austria | HPLC Columns Purification and Analysis of Proteins and Peptides

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Mittlere Strasse 69

CH-4056 Basel


Tel./fax:     +41 61 381 5022

Mobile:      +41 78 813 5183

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NEW!  HIC-MS for Top-Down Proteomics


Our new series of materials for HIC (Hydrophobic Interaction Chromatography) permit the retention of proteins using concentrations of ammonium acetate that are compatible with direct analysis via mass spectrometry. Proteins can be separated and eluted with their native structures intact. This is useful for top-down proteomics and permits the analysis of some proteins that are not compatible with the conditions of reversed-phase chromatography. The example below shows base peak chromatograms of some protein standards, their mass spectral charge state patterns, and isotopic resolution in the deconvoluted mass spectra. Note the separation of aprotinin from an oxidized variant.



Column: PolyHEPTYL ATM capillary, 100 x 0.2 mm, 3-μm, 1500-Å

Mobile Phase: A) 1M ammonium acetate; B) 20 mM ammonium acetate with 50% ACN

Gradient: 15 min linear, 0-100% B, then 5 min at 100% B

Flow: 2.4 μl/min

Detection: maXisTM Plus Q-TOF mass spectrometer (Bruker Daltonics)