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PolyLC Switzerland, Europe, Germany, Austria | HPLC Columns Purification and Analysis of Proteins and Peptides

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Mittlere Strasse 69

CH-4056 Basel

Switzerland.

Tel./fax:     +41 61 381 5022

Mobile:      +41 78 813 5183

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URL:         www.chromatechnologies.com

 

Separation and analysis of hemoglobin variants with PolyCAT A

 

PolyCAT ATM ia the gold standard worldwide for analysis of hemoglobin variants by cation-exchange HPLC. The most common variants can be separated in 3.5 minutes with good quantitation and with a turnaround time between samples of 4 - 4.5 minutes. This includes quantitation of Hb A1c for diagnosis of diabetes. The examples below were obtained with the widely-used 35 x 4.6 mm column packed with the 3-μm, 1500-Å pore version of PolyCAT A (item 3.54CT0315).

 

 

These variants were analyzed with a slightly longer column to improve separation.

Column: PolyCAT A, 50 x 4.6 mm, 3-μm, 1500-Å (item# 054CT0315).

 

 

 

 

Hemoglobin F, A, A2, S, C standard

Column: PolyCAT A, 35 x 4.6 mm, 3-μm, 1500-Å (item 3.54CT0315)

Solvent A: 20 mM bis-Tris + 2 mM KCN, pH 6.8

Solvent B: 20 mM bis-Tris + 2 mM KCN + 200 mM NaCl, pH 6.8

Gradient: 0-8 min, 2-25% B; 8.1-18 min, 25-100% B.

Flow rate: 1.5 ml/min.

 

 

Order from:

CHROMATOGRAPHIC TECHNOLOGIES

Mittlere Strasse 69

CH-4056 Basel

Switzerland.

Tel./fax:     +41 61 381 5022

Mobile:      +41 78 813 5183

Email:       This email address is being protected from spambots. You need JavaScript enabled to view it.

URL:         www.chromatechnologies.com